Abstract: Ultrasound-guided percutaneous lung needle biopsy (US-PLNB) is a pivotal diagnostic technique for subpleural pulmonary lesions, offering significant advantages such as real-time imaging, absence of ionizing radiation, and high operational flexibility. To standardize its clinical application and enhance both procedural safety and diagnostic accuracy, a multidisciplinary panel of experts was convened by eleven authoritative Chinese academic societies, including “Chinese Society of Ultrasound in Medicine”. Based on the latest domestic and international evidence-based medicine, this panel developed Chinese expert consensus on ultrasound-guided percutaneous lung needle biopsy (2025 edition). This consensus systematically elaborates on the indications and contraindications, preprocedural assessment and preparation, intraoperative techniques, complication prevention and management, and post-procedural care for US-PLNB. Furthermore, it puts forward 18 specific evidence-based recommendations. The primary aim of this consensus is to provide radiologists and clinicians with standardized operational guidance, promote the standardized application of US-PLNB, and ultimately improve patient care for individuals with pulmonary diseases.